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The nitrocellulose membranes were immunoblotted with primary antibodies against acyl-CoA synthetase long-chain family member 4 (ACSL4) (1:1000, Santa cruz), Bax (1:1000, Cell signaling), Bcl-2 (1:1000, Cell signaling), DRP1 (1:1000, Cell signaling), GAPDH (1:5000 Abcam), GPX4 (1:1000, Abcam), MLKL (1:1000, Invitrogen), OPA1 (1:1000, Cell signaling), pDRP1 (Ser616) (1:1000, Cell signaling), pRIPK3 (Ser232) (1:1000, Cell signaling), and RIPK3 (1:1000, Cell signaling) overnight at 4 C, and then incubated with the appropriate secondary antibody (1:1000, Santa cruz)

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Alba M, Fintini D, Sagazio A, Lawrence B, Castaigne JP, Frohman LA, Salvatori R Once-daily administration of CJC-1295, a long-acting growth hormone-releasing hormone (GHRH) analog, normalizes growth in the GHRH knockout mouse